Author: Nelly Mostajo Berrospi; Marie Lataretu; Sebastian Krautwurst; Florian Mock; Daniel Desirò; Kevin Lamkiewicz; Maximilian Collatz; Andreas Schoen; Friedemann Weber; Manja Marz; Martin Hölzer
Title: A comprehensive annotation and differential expression analysis of short and long non-coding RNAs in 16 bat genomes Document date: 2019_8_19
ID: ihqvcxv6_8
Snippet: In this study, we comprehensively annotated non-coding RNAs in 16 available bat genome assemblies (Tab. 1). For each bat species, we provide final annotations that are compatible with current Ensembl and NCBI (National Center for Biotechnology Information) standards (GTF format) and that can be directly used in other studies, for example for differential gene expression analysis. We compare our new annotations with the currently available annotat.....
Document: In this study, we comprehensively annotated non-coding RNAs in 16 available bat genome assemblies (Tab. 1). For each bat species, we provide final annotations that are compatible with current Ensembl and NCBI (National Center for Biotechnology Information) standards (GTF format) and that can be directly used in other studies, for example for differential gene expression analysis. We compare our new annotations with the currently available annotations for bats and show that a large number of non-coding genes are simply not annotated and are therefore overlooked by other studies. We used six RNA-Seq data sets comprising different conditions (Tab. 2) to validate our annotations and to determine the expression levels of our newly annotated ncRNAs. Exemplarily, we show that our novel annotations can be used to identify ncRNAs that are significantly differential expressed during viral infections and were missed by previous studies.
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