Selected article for: "mutant wild type and wild type"
Author: Elisa Oberbeckmann; Vanessa Niebauer; Shinya Watanabe; Lucas Farnung; Manuela Moldt; Andrea Schmid; Patrick Cramer; Craig L. Peterson; Sebastian Eustermann; Karl-Peter Hopfner; Philipp Korber
Title: Ruler elements in chromatin remodelers set nucleosome array spacing and phasing
  • Document date: 2020_2_29
    • ID: 5hkd80eh_31
    Snippet: The pGP546 yeast genomic plasmid library was expanded from the clonal plates provided by Open Biosystems. For generation of genomic plasmid libraries, the S. pombe strain Hu0303 (Ekwall group) and E. coli strain (ATCC 11303 strain, 14380, Affymetrix) were used. INO80 wild-type and mutant complexes, Chd1 and FACT were expressed in Trichoplusiani insect cells. Spodoptera frugiperda sf21 insect cells were used for virus production. The Saccharomyces.....
    Document: The pGP546 yeast genomic plasmid library was expanded from the clonal plates provided by Open Biosystems. For generation of genomic plasmid libraries, the S. pombe strain Hu0303 (Ekwall group) and E. coli strain (ATCC 11303 strain, 14380, Affymetrix) were used. INO80 wild-type and mutant complexes, Chd1 and FACT were expressed in Trichoplusiani insect cells. Spodoptera frugiperda sf21 insect cells were used for virus production. The Saccharomyces cerevisiae ISW1a and Fun30 remodelers were purified from the correspondingly TAP-tagged yeast strains, Ioc3-TAP, Fun30-Tap, as provided by Open Biosystems. Yeast ISW2 was purified from strain YTT480 (ISW2-2xFLAG, Tsukiyama et al., 1999) . Reb1 was purified from E. coli BL21 (DE3) cd+ cells. The Drosophila embryo histones were prepared from the Drosophila melanogaster strain OregonR.

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