Reverse transcription loop-mediated isothermal amplification combined with nanoparticles-based biosensor for diagnosis of COVID-19

Xiong Zhu; Xiaoxia Wang; Limei Han; Ting Chen; Licheng Wang; Huan Li; Sha Li; Lvfen He; Xiaoying Fu; Shaojin Chen; Xing Mei; Hai Chen; Yi Wang

The copyright holder for this preprint . https://doi.org/10.1101/2020.03.17.20037796 doi: medRxiv preprint reagents (e.g., PH indicator) and expensive instrument (e.g., real-time turbidity) (13, F1ab) (13, 14) . 250 The data of analytical sensitivity validated that RT-LAMP-NBS assay is 251 sufficiently sensitive for diagnosis of COVID-19. Us this protocol, detection limit of 252 COVID-19 RT-LAMP-NBS was 12 copies each of F1ab-plasmid and np-plasmid, 253 which is in conformity with assay's sensitivity generated from F1ab-RT-LAMP-NBS 254 and N-RT-LAMP-NBS detection (Figure 4, S4 and S5) . The COVID-19 255 RT-LAMP-NBS assay did not improve or decrease the analytical sensitivity when is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.