Selected article for: "assay dynamic range and dynamic range"
Author: Xiaotian Tan; Cory Lin; Jie Zhang; Maung Kyaw Khaing Oo; Xudong Fan
Title: Rapid and quantitative detection of COVID-19 markers in micro-liter sized samples
  • Document date: 2020_4_22
    • ID: 0tdfvlqd_34
    Snippet: Same as in the IgG detection experiment, we performed a side-by-side study with the S1 proteins from SARS-CoV-2 and SARS-CoV. To mimic actual clinical settings, we used 10 times diluted human serum as the solvent of the S1 protein, as we do not expect to see a high concentration of viral S1 protein in serum (or saliva). The entire dynamic range of the S1 detection assay is presented in Fig. 3(B) . The linear dynamic range for SARS-CoV-2 S1 and SA.....
    Document: Same as in the IgG detection experiment, we performed a side-by-side study with the S1 proteins from SARS-CoV-2 and SARS-CoV. To mimic actual clinical settings, we used 10 times diluted human serum as the solvent of the S1 protein, as we do not expect to see a high concentration of viral S1 protein in serum (or saliva). The entire dynamic range of the S1 detection assay is presented in Fig. 3(B) . The linear dynamic range for SARS-CoV-2 S1 and SARS-CoV S1 is 0.4-1000 ng/mL and 0.2-250 ng/mL with a slope of 0.89 and 0.92 (in the log-log scale), respectively. According to Figures 3(B) and (C), the detection of SARS-CoV S1 appears to have a higher sensitivity than SARS-CoV-2 S1. This is because both of antibodies (D001 and D003) used in this assay were originally raised against the RBD of SARS-CoV S1. A higher sensitivity in detecting SARS-CoV-2 S1 protein may be achieved in the future with the antibodies specifically developed against the S1 protein (or S1 RBD) of SARS-CoV-2.

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