Replication of Equine arteritis virus is efficiently suppressed by purine and pyrimidine biosynthesis inhibitors

jose-carlos Valle-Casuso; Delphine Gaudaire; Lydie Martin-Faivre; anthony madeline; Patrick Dallemagne; Stephane Pronost; Helene Munier-Lehmann; Stephan Zientara; Pierre-Olivier Vidalain; Aymeric Hans

were not toxic for ED cells at the tested concentrations up to 100 µg/ml. Among the three molecules, The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.10.035402 doi: bioRxiv preprint infection model that is fully compatible with HTS and can be automated. Because of its flexibility, our 265 assay is appropriate for the screening of compound libraries or biological products (serum, 266 nanobodies, cytokines, etc) and will allow the exploration of treatment combinations against EAV 267 infection. Thanks to this assay, we demonstrated that ribavirin effectively reduces the replication of 268 EAV in horse cells at concentrations that are not toxic. This is supports reports showing that ribavirin 269 efficiently inhibits the replication of multiple nidoviruses from both the Coronaviridae and Arteriviridae 270 family such as the MERS-CoV 33 and the PPRSV 28 , respectively. Additionally, we showed that the 271 DHODH inhibitors GAC50 and IPPA17-A04 also impair the replication of EAV, with IPPA17-A04 272 outperforming ribavirin and GAC50 as assessed by a higher selectivity index. It is known that America. Equine practice 9, 295-309 (1993). 359 author/funder. All rights reserved. No reuse allowed without permission.