N-terminal domain antigenic mapping reveals a site of vulnerability for SARS-CoV-2

McCallum, Matthew; Marco, Anna De; Lempp, Florian; Tortorici, M. Alejandra; Pinto, Dora; Walls, Alexandra C.; Beltramello, Martina; Chen, Alex; Liu, Zhuoming; Zatta, Fabrizia; Zepeda, Samantha; di Iulio, Julia; Bowen, John E.; Montiel-Ruiz, Martin; Zhou, Jiayi; Rosen, Laura E.; Bianchi, Siro; Guarino, Barbara; Fregni, Chiara Silacci; Abdelnabi, Rana; Caroline Foo, Shi-Yan; Rothlauf, Paul W.; Bloyet, Louis-Marie; Benigni, Fabio; Cameroni, Elisabetta; Neyts, Johan; Riva, Agostino; Snell, Gyorgy; Telenti, Amalio; Whelan, Sean P.J.; Virgin, Herbert W.; Corti, Davide; Pizzuto, Matteo Samuele; Veesler, David

SARS-CoV-2 entry into host cells is orchestrated by the spike (S) glycoprotein that contains an immunodominant receptor-binding domain (RBD) targeted by the largest fraction of neutralizing antibodies (Abs) in COVID-19 patient plasma. Little is known about neutralizing Abs binding to epitopes outside the RBD and their contribution to protection. Here, we describe 41 human monoclonal Abs (mAbs) derived from memory B cells, which recognize the SARS-CoV-2 S N-terminal domain (NTD) and show that a subset of them neutralize SARS-CoV-2 ultrapotently. We define an antigenic map of the SARS-CoV-2 NTD and identify a supersite recognized by all known NTD-specific neutralizing mAbs. These mAbs inhibit cell-to-cell fusion, activate effector functions, and protect Syrian hamsters from SARS-CoV-2 challenge. SARS-CoV-2 variants, including the 501Y.V2 and B.1.1.7 lineages, harbor frequent mutations localized in the NTD supersite suggesting ongoing selective pressure and the importance of NTD-specific neutralizing mAbs to protective immunity.