Author: Yueting Zhang; Gary Whittaker
Title: Productive infection of field strains of avian coronavirus infectious bronchitis virus in chicken peripheral blood-derived monocyte Document date: 2016_2_26
ID: lcpp5fim_5
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/041558 doi: bioRxiv preprint >200 cells quantified from three independent experiments. For RT--PCR for viral detection, 10 6 PBMCs were infected with 7x10 2 TCID50 IBV--M41. At indicated time points, culture supernatant was collected and filtered. Viral RNA was extracted from the supernatant using a Qiagen Viral RNA extraction kit......
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/041558 doi: bioRxiv preprint >200 cells quantified from three independent experiments. For RT--PCR for viral detection, 10 6 PBMCs were infected with 7x10 2 TCID50 IBV--M41. At indicated time points, culture supernatant was collected and filtered. Viral RNA was extracted from the supernatant using a Qiagen Viral RNA extraction kit. The RT--PCR reaction used a SuperScript One--
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