Author: Nila Roy Choudhury; Gregory Heikel; Maryia Trubitsyna; Peter Kubik; Jakub Stanislaw Nowak; Shaun Webb; Sander Granneman; Christos Spanos; Juri Rappsilber; Alfredo Castello; Gracjan Michlewski
Title: RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination Document date: 2017_10_9
ID: ifla4aix_71
Snippet: The incubation was followed by a series of washes with buffer G (20 mM Tris [pH 7.5], 135 mM NaCl, 1.5 mM MgCl2, 10% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol, and 0.2 mM phenylmethanesulfonylfluoride). After the final wash, the proteins associated with the beads were analyzed by western blotting......
Document: The incubation was followed by a series of washes with buffer G (20 mM Tris [pH 7.5], 135 mM NaCl, 1.5 mM MgCl2, 10% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol, and 0.2 mM phenylmethanesulfonylfluoride). After the final wash, the proteins associated with the beads were analyzed by western blotting.
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