Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells

Hualong Xiong; Yangtao Wu; Jiali Cao; Ren Yang; Jian Ma; Xiaoyang Qiao; Xiangyang Yao; Baohui Zhang; Yali Zhang; Wangheng Hou; Yang Shi; Jingjing Xu; Liang Zhang; Shaojuan Wang; Baorong Fu; Ting Yang; Shengxiang Ge; Jun Zhang; Quan Yuan; Baoying Huang; Zhiyong Li; Tianying Zhang; Ningshao Xia

The inhibition rates of mAb and serum were calculated according to the decrease of GFP positive cell number (for pseudotype-based neutralization assay) or decrease of . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.08.026948 doi: bioRxiv preprint RNA levels (for live SARS-CoV-2-based neutralization assay). The IC50 (the half maximal inhibitory concentration) or ID 50 (plasma/serum dilutions causing a 50% reduction of GFP-positive cell number or RNA level) values were calculated with non-linear regression, i.e. log (inhibitor) vs. normalized response -Variable slope, using GraphPad Prism 7.00 (GraphPad Software, Inc., San Diego, CA, USA). The correlation between analysis result of pseudovirus system and live virus system was analysed by linear regression using GraphPad Prism 7.00.