Author: Hualong Xiong; Yangtao Wu; Jiali Cao; Ren Yang; Jian Ma; Xiaoyang Qiao; Xiangyang Yao; Baohui Zhang; Yali Zhang; Wangheng Hou; Yang Shi; Jingjing Xu; Liang Zhang; Shaojuan Wang; Baorong Fu; Ting Yang; Shengxiang Ge; Jun Zhang; Quan Yuan; Baoying Huang; Zhiyong Li; Tianying Zhang; Ningshao Xia
Title: Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells Document date: 2020_4_9
ID: fvjh3g7v_22
Snippet: VSVdG-SARS-CoV-2-Sdel18 based system was used to analyze the neutralizing antibodies in the serum of 18 convalescent patients. The serum was diluted 60-fold as the first concentration, and then prepared doubling dilutions (60, 120, 240, etc.). BHK21-hACE2 cell was seeded and incubated with the mixture of gradient diluted serum and VSVdG-SARS-CoV-2-Sdel18 virus (MOI=0.05). The GFP positive cell number was counted to analysed the neutralizing activ.....
Document: VSVdG-SARS-CoV-2-Sdel18 based system was used to analyze the neutralizing antibodies in the serum of 18 convalescent patients. The serum was diluted 60-fold as the first concentration, and then prepared doubling dilutions (60, 120, 240, etc.). BHK21-hACE2 cell was seeded and incubated with the mixture of gradient diluted serum and VSVdG-SARS-CoV-2-Sdel18 virus (MOI=0.05). The GFP positive cell number was counted to analysed the neutralizing activity of serum ( Figure 6A ).
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