Author: Hualong Xiong; Yangtao Wu; Jiali Cao; Ren Yang; Jian Ma; Xiaoyang Qiao; Xiangyang Yao; Baohui Zhang; Yali Zhang; Wangheng Hou; Yang Shi; Jingjing Xu; Liang Zhang; Shaojuan Wang; Baorong Fu; Ting Yang; Shengxiang Ge; Jun Zhang; Quan Yuan; Baoying Huang; Zhiyong Li; Tianying Zhang; Ningshao Xia
Title: Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells Document date: 2020_4_9
ID: fvjh3g7v_6
Snippet: coronavirus et al., in which the VSV G gene is deleted (VSVdG) and the gene encoding GFP, luciferase or other reporter genes was integrated [10] [11] [12] . Pseudotyped viruses provide a safe viral entry model because of their inability to produce infectious progeny virus. VSV assembly occurs at the plasma membrane and involves budding of virions from the cell surface. During budding, VSV acquires an envelope consisting of a lipid bilayer derived.....
Document: coronavirus et al., in which the VSV G gene is deleted (VSVdG) and the gene encoding GFP, luciferase or other reporter genes was integrated [10] [11] [12] . Pseudotyped viruses provide a safe viral entry model because of their inability to produce infectious progeny virus. VSV assembly occurs at the plasma membrane and involves budding of virions from the cell surface. During budding, VSV acquires an envelope consisting of a lipid bilayer derived from the plasma membrane and spike proteins consisting of trimers of the VSV glycoprotein (VSV-G). When the VSV-G is absence and the glycoprotein from heterologous virus is complacently expressed in cells infected with rVSV-dG, the glycoprotein of heterologous virus could be assembled into the VSV membrane.
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