Author: Liao, Y.; Yuan, Q.; Torres, J.; Tam, J.P.; Liu, D.X.
Title: Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein Cord-id: u6v6bxwj Document date: 2006_6_5
ID: u6v6bxwj
Snippet: A diverse group of cytolytic animal viruses encodes small, hydrophobic proteins to modify host cell membrane permeability to ions and small molecules during their infection cycles. In this study, we show that expression of the SARS-CoV E protein in mammalian cells alters the membrane permeability of these cells. Immunofluorescent staining and cell fractionation studies demonstrate that this protein is an integral membrane protein. It is mainly localized to the ER and the Golgi apparatus. The pro
Document: A diverse group of cytolytic animal viruses encodes small, hydrophobic proteins to modify host cell membrane permeability to ions and small molecules during their infection cycles. In this study, we show that expression of the SARS-CoV E protein in mammalian cells alters the membrane permeability of these cells. Immunofluorescent staining and cell fractionation studies demonstrate that this protein is an integral membrane protein. It is mainly localized to the ER and the Golgi apparatus. The protein can be translocated to the cell surface and is partially associated with lipid rafts. Further biochemical characterization of the protein reveals that it is posttranslationally modified by palmitoylation on all three cysteine residues. Systematic mutagenesis studies confirm that the membrane permeabilizing activity of the SARS-CoV E protein is associated with its transmembrane domain.
Search related documents:
Co phrase search for related documents- absence presence and localization pattern: 1
- absence presence and low concentration: 1, 2, 3
- absence presence and low density: 1, 2, 3
- absence presence and low expression: 1, 2, 3
- absence presence and lysis buffer: 1, 2, 3, 4, 5
- absence presence and lysis buffer sucrose: 1
- acute respiratory syndrome and localization pattern: 1
- acute respiratory syndrome and low concentration: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52
- acute respiratory syndrome and low density: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30
- acute respiratory syndrome and low expression: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45
- acute respiratory syndrome and lysis buffer: 1, 2, 3
- loading buffer and lysis buffer: 1, 2, 3, 4, 5, 6
- localization pattern and low expression: 1
- low concentration and lysis buffer: 1, 2
- low density and lysis buffer: 1
Co phrase search for related documents, hyperlinks ordered by date