Selected article for: "luciferase expression and protein expression"
Author: Carbajo-Lozoya, Javier; Müller, Marcel A.; Kallies, Stephan; Thiel, Volker; Drosten, Christian; von Brunn, Albrecht
Title: Replication of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E is inhibited by the drug FK506
  • Cord-id: xanqvdhn
  • Document date: 2012_2_10
    • ID: xanqvdhn
    Snippet: Recent research has shown that Coronavirus (CoV) replication depends on active immunophilin pathways. Here we demonstrate that the drug FK506 (Tacrolimus) inhibited strongly the growth of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E at low, non-cytotoxic concentrations in cell culture. As shown by plaque titration, qPCR, Luciferase- and green fluorescent protein (GFP) reporter gene expression, replication was diminished by several orders of magnitude. Knockdown of the cellular FK506-bin
    Document: Recent research has shown that Coronavirus (CoV) replication depends on active immunophilin pathways. Here we demonstrate that the drug FK506 (Tacrolimus) inhibited strongly the growth of human coronaviruses SARS-CoV, HCoV-NL63 and HCoV-229E at low, non-cytotoxic concentrations in cell culture. As shown by plaque titration, qPCR, Luciferase- and green fluorescent protein (GFP) reporter gene expression, replication was diminished by several orders of magnitude. Knockdown of the cellular FK506-binding proteins FKBP1A and FKBP1B in CaCo2 cells prevented replication of HCoV-NL63, suggesting the requirement of these members of the immunophilin family for virus growth.

    Search related documents:
    Co phrase search for related documents
    • activity influence and luciferase activity: 1
    • acute respiratory syndrome sars cov coronavirus and log percentage: 1
    • acute respiratory syndrome sars cov coronavirus and log reduction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • acute respiratory syndrome sars cov coronavirus and low micromolar: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
    • acute respiratory syndrome sars cov coronavirus and low micromolar concentration: 1
    • acute respiratory syndrome sars cov coronavirus and low micromolar range: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • acute respiratory syndrome sars cov coronavirus and luciferase activity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • log reduction and low micromolar: 1, 2