Author: Laura E Lamb; Sarah N Bartolone; Elijah Ward; Michael B Chancellor
Title: Rapid Detection of Novel Coronavirus (COVID-19) by Reverse Transcription-Loop-Mediated Isothermal Amplification Document date: 2020_2_24
ID: 6kpgt70s_14
Snippet: To demonstrate the clinical utility of this system for COVID-19 detection, we spiked various human specimens with COVID-19, Middle East Respiratory Syndrome (MERS), Betacoronavirus England-1 (BtCoV), or Murine hepatitis virus (MHV). MERS, BtCoV and MHV spiked samples were used to test the specificity of the RT-LAMP assay. Spiked specimens included serum, urine, saliva, oropharyngeal swabs, and nasopharyngeal swabs as we wanted to verify that subs.....
Document: To demonstrate the clinical utility of this system for COVID-19 detection, we spiked various human specimens with COVID-19, Middle East Respiratory Syndrome (MERS), Betacoronavirus England-1 (BtCoV), or Murine hepatitis virus (MHV). MERS, BtCoV and MHV spiked samples were used to test the specificity of the RT-LAMP assay. Spiked specimens included serum, urine, saliva, oropharyngeal swabs, and nasopharyngeal swabs as we wanted to verify that substances present in these samples would not interfere with RT-LAMP. Samples were directly used for RT-LAMP without performing nucleic acid All rights reserved. No reuse allowed without permission.
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