Author: Soon Keong Wee; Suppiah Paramalingam Sivalingam; Eric Peng Huat Yap
Title: Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler Document date: 2020_4_20
ID: e1oinu71_32
Snippet: Quantitative RT-PCR of SARS-CoV-2 RNA is currently the method of choice for the diagnosis of Covid-19. In our DIRECT-PCR method, we used inhibitor-resistant enzymes and reagents to eliminate the RNA extraction step. The use of synthetic RNA as template obviated the need to handle viral nucleic acids. With fast DIRECT-PCR protocol, we achieved further reduction in assay time by optimizing the thermocycling conditions. We also validated the analyti.....
Document: Quantitative RT-PCR of SARS-CoV-2 RNA is currently the method of choice for the diagnosis of Covid-19. In our DIRECT-PCR method, we used inhibitor-resistant enzymes and reagents to eliminate the RNA extraction step. The use of synthetic RNA as template obviated the need to handle viral nucleic acids. With fast DIRECT-PCR protocol, we achieved further reduction in assay time by optimizing the thermocycling conditions. We also validated the analytical performance of DIRECT-PCR on spiked crude samples and demonstrated its use on a portable platform.
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