CD8(+)PD-L1(+)CXCR3(+) polyfunctional T cell abundances are associated with survival in critical SARS-CoV-2–infected patients

Adam, Lucille; Rosenbaum, Pierre; Quentric, Paul; Parizot, Christophe; Bonduelle, Olivia; Guillou, Noëlline; Corneau, Aurélien; Dorgham, Karim; Miyara, Makoto; Luyt, Charles-Edouard; Guihot, Amélie; Gorochov, Guy; Combadière, Christophe; Combadière, Behazine

The importance of the adaptive T cell response in the control and resolution of viral infection has been well established. However, the nature of T cell–mediated viral control mechanisms in life-threatening stages of COVID-19 has yet to be determined. The aim of the present study was to determine the function and phenotype of T cell populations associated with survival or death of patients with COVID-19 in intensive care as a result of phenotypic and functional profiling by mass cytometry. Increased frequencies of circulating, polyfunctional CD4(+)CXCR5(+)HLA-DR(+) stem cell memory T cells (Tscms) and decreased proportions of granzyme B–expressing and perforin-expressing effector memory T cells were detected in recovered and deceased patients, respectively. The higher abundance of polyfunctional PD-L1(+)CXCR3(+)CD8(+) effector T cells (Teffs), CXCR5(+)HLA-DR(+) Tscms, and anti-nucleocapsid (anti-NC) cytokine-producing T cells permitted us to differentiate between recovered and deceased patients. The results from a principal component analysis show an imbalance in the T cell compartment that allowed for the separation of recovered and deceased patients. The paucity of circulating PD-L1(+)CXCR3(+)CD8(+) Teffs and NC-specific CD8(+) T cells accurately forecasts fatal disease outcome. This study provides insight into the nature of the T cell populations involved in the control of COVID-19 and therefore might impact T cell–based vaccine designs for this infectious disease.