Author: Xiong Zhu; Xiaoxia Wang; Limei Han; Ting Chen; Licheng Wang; Huan Li; Sha Li; Lvfen He; Xiaoying Fu; Shaojin Chen; Xing Mei; Hai Chen; Yi Wang
Title: Reverse transcription loop-mediated isothermal amplification combined with nanoparticles-based biosensor for diagnosis of COVID-19 Document date: 2020_3_20
ID: fq11rhab_15
Snippet: The copyright holder for this preprint . https://doi.org/10.1101/2020.03.17.20037796 doi: medRxiv preprint Two RT-LAMP primer sets (F1ab-RT-LAMP and np-RT-LAMP) were designed 294 according the LAMP mechanism using a specialized software (PrimerExplore V5), 295 which targeted F1ab and np gene of SARS-CoV-2 (GenBank MN908947, 296 Wuhan-Hu-1) (Figure 6) . Then, a Blast analysis of the GenBank nucleotide database 297 was performed for the F1ab-and np.....
Document: The copyright holder for this preprint . https://doi.org/10.1101/2020.03.17.20037796 doi: medRxiv preprint Two RT-LAMP primer sets (F1ab-RT-LAMP and np-RT-LAMP) were designed 294 according the LAMP mechanism using a specialized software (PrimerExplore V5), 295 which targeted F1ab and np gene of SARS-CoV-2 (GenBank MN908947, 296 Wuhan-Hu-1) (Figure 6) . Then, a Blast analysis of the GenBank nucleotide database 297 was performed for the F1ab-and np-LAMP primers to validate sequence specificity.
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