Author: Ragan, Katherine B.; Bhadra, Sanchita; Choi, Joon H.; Towers, Dalton; Sullivan, Christopher S.; Ellington, Andrew D.
Title: Comparison of media and standards for SARS-CoV-2 RT-qPCR without prior RNA preparation Cord-id: w7wuqgad Document date: 2020_9_17
ID: w7wuqgad
Snippet: Since the emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, there have been demands on the testing infrastructure that have strained testing capacity. As a simplification of method, we confirm the efficacy of RNA extraction-free RT-qPCR and saline as an alternative patient sample storage buffer. In addition, amongst potential reagent shortages, it has sometimes been difficult to obtain inactivated viral particles. We have therefore also characterized armored
Document: Since the emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, there have been demands on the testing infrastructure that have strained testing capacity. As a simplification of method, we confirm the efficacy of RNA extraction-free RT-qPCR and saline as an alternative patient sample storage buffer. In addition, amongst potential reagent shortages, it has sometimes been difficult to obtain inactivated viral particles. We have therefore also characterized armored SARS-CoV-2 RNA from Asuragen as an alternative diagnostic standard to ATCC genomic SARS-CoV-2 RNA and heat inactivated virions and provide guidelines for its use in RT-qPCR.
Search related documents:
Co phrase search for related documents- absence presence and low sensitivity: 1, 2, 3, 4, 5, 6, 7
Co phrase search for related documents, hyperlinks ordered by date