Author: Chunyun Sun; Long Chen; Ji Yang; Chunxia Luo; Yanjing Zhang; Jing Li; Jiahui Yang; Jie Zhang; Liangzhi Xie
Title: SARS-CoV-2 and SARS-CoV Spike-RBD Structure and Receptor Binding Comparison and Potential Implications on Neutralizing Antibody and Vaccine Development Document date: 2020_2_20
ID: nhq0oq8y_55
Snippet: Indicated proteins were coated on 96 well plates using CBS buffer over night at 4℃. BSA was used for blocking at room temperature for 1h. Indicated corresponding proteins or antibodies were then added and incubated at room temperature for 1h. After washing away the unbound proteins or antibodies, secondary antibody with HRP labeling were added and incubated for another hour before washed away. Developing buffer was added and incubated for 5-30 .....
Document: Indicated proteins were coated on 96 well plates using CBS buffer over night at 4℃. BSA was used for blocking at room temperature for 1h. Indicated corresponding proteins or antibodies were then added and incubated at room temperature for 1h. After washing away the unbound proteins or antibodies, secondary antibody with HRP labeling were added and incubated for another hour before washed away. Developing buffer was added and incubated for 5-30 min, 1% H 2 SO 4 was added to stop the reaction and absorbance at 450nm was detected with a microplate reader.
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