Author: Renfei Lu; Jian Wang; Min Li; Yaqi Wang; Jia Dong; Weihua Cai
Title: SARS-CoV-2 detection using digital PCR for COVID-19 diagnosis, treatment monitoring and criteria for discharge Document date: 2020_3_30
ID: mskkahwi_20
Snippet: The samples with ORF 1ab positive detected in blood and sputum for dPCR is 1 more than RT-PCR (1 220 vs 0). While the samples with ORF1ab positive detected in stool for dPCR is 2 less than RT-PCR (2 vs 221 4). Therefore, dPCR showed less sensitivity than RT-PCR in specimens from stool, higher sensitivity 222 than RT-PCR in specimens from throat, blood and sputum. Different detection sensitivity of dPCR 223 assay for different sample types may req.....
Document: The samples with ORF 1ab positive detected in blood and sputum for dPCR is 1 more than RT-PCR (1 220 vs 0). While the samples with ORF1ab positive detected in stool for dPCR is 2 less than RT-PCR (2 vs 221 4). Therefore, dPCR showed less sensitivity than RT-PCR in specimens from stool, higher sensitivity 222 than RT-PCR in specimens from throat, blood and sputum. Different detection sensitivity of dPCR 223 assay for different sample types may require further experiments to draw a conclusion. 224
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