Author: Lu Guo; Xuehan Sun; Xinge Wang; Chen Liang; Haiping Jiang; Qingqin Gao; Moyu Dai; Bin Qu; Sen Fang; Yihuan Mao; Yangcan Chen; Guihai Feng; Qi Gu; Liu Wang; Ruiqi Rachel Wang; Qi Zhou; Wei Li
Title: SARS-CoV-2 detection with CRISPR diagnostics Document date: 2020_4_11
ID: 2una9767_13
Snippet: Thus, to investigate the virus handling processes, we produced pseudoviruses by packaging the target sequences of SARS-CoV-2, SARS-CoV, MERS-CoV into actual lentivirus particles (Supplementary information, Fig. S8 ). These pseudoviruses were diluted serially and treated with either virus genome extraction kits (spin column) or lysis buffer, respectively. Our results demonstrated that the spin column treatment gave a lower detection limit of 1 × .....
Document: Thus, to investigate the virus handling processes, we produced pseudoviruses by packaging the target sequences of SARS-CoV-2, SARS-CoV, MERS-CoV into actual lentivirus particles (Supplementary information, Fig. S8 ). These pseudoviruses were diluted serially and treated with either virus genome extraction kits (spin column) or lysis buffer, respectively. Our results demonstrated that the spin column treatment gave a lower detection limit of 1 × 10 4 copies/mL (Fig. 1e) . On the other hand, the lysis buffer offered higher usability, but raised the author/funder. All rights reserved. No reuse allowed without permission.
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