Selected article for: "flow rate and Îl flow rate"
Author: Ke Wang; Wei Chen; Yu-Sen Zhou; Jian-Qi Lian; Zheng Zhang; Peng Du; Li Gong; Yang Zhang; Hong-Yong Cui; Jie-Jie Geng; Bin Wang; Xiu-Xuan Sun; Chun-Fu Wang; Xu Yang; Peng Lin; Yong-Qiang Deng; Ding Wei; Xiang-Min Yang; Yu-Meng Zhu; Kui Zhang; Zhao-Hui Zheng; Jin-Lin Miao; Ting Guo; Ying Shi; Jun Zhang; Ling Fu; Qing-Yi Wang; Huijie Bian; Ping Zhu; Zhi-Nan Chen
Title: SARS-CoV-2 invades host cells via a novel route: CD147-spike protein
  • Document date: 2020_3_14
    • ID: 5hpbjkft_23
    Snippet: SPR analysis was performed by BIAcore 3000 system (BIAcore). His-CD147 (produced by our laboratory) was fixed to the surface of CM5 sensor chips (GE Healthcare Bio-Sciences AB) by amino coupling kit (GE Healthcare, BR-1000-50); interaction between CD147 and SP (RBD) (GenScript, China) was detected using Kinetic Analysis/Concentration Series/Direct Binding mode, and the flow rate was set to 15 μl/min, both binding time and dissociation time were .....
    Document: SPR analysis was performed by BIAcore 3000 system (BIAcore). His-CD147 (produced by our laboratory) was fixed to the surface of CM5 sensor chips (GE Healthcare Bio-Sciences AB) by amino coupling kit (GE Healthcare, BR-1000-50); interaction between CD147 and SP (RBD) (GenScript, China) was detected using Kinetic Analysis/Concentration Series/Direct Binding mode, and the flow rate was set to 15 μl/min, both binding time and dissociation time were 3 min. The results were analyzed by BIAevaluation software to determine the affinity constant.

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