Author: NISREEN M.A. OKBA; Marcel A Muller; Wentao Li; Chunyan Wang; Corine H. GeurtsvanKessel; Victor M. Corman; Mart M. Lamers; Reina S. Sikkema; Erwin de Bruin; Felicity D. Chandler; Yazdan Yazdanpanah; Quentin Le Hingrat; Diane Descamps; Nadhira Houhou-Fidouh; Chantal B. E. M. Reusken; Berend-Jan Bosch; Christian Drosten; Marion P.G. Koopmans; Bart L. Haagmans
Title: SARS-CoV-2 specific antibody responses in COVID-19 patients Document date: 2020_3_20
ID: 9595vm0k_16
Snippet: The PRNT for the German sera was done as described previously using Vero E6 cells After 1 hour at 37°C the supernatants were discarded, the cells were washed once with PBS and supplemented with 1.2% Avicel solution in DMEM. After 3 days at plates were fixed and inactivated using a 6% formaldehyde/PBS solution and stained with crystal violet......
Document: The PRNT for the German sera was done as described previously using Vero E6 cells After 1 hour at 37°C the supernatants were discarded, the cells were washed once with PBS and supplemented with 1.2% Avicel solution in DMEM. After 3 days at plates were fixed and inactivated using a 6% formaldehyde/PBS solution and stained with crystal violet.
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