Author: Salman L. Butt; Eric C. Erwood; Jian Zhang; Holly S. Sellers; Kelsey Young; Kevin K. Lahmers; James B. Stanton
Title: Real-time, MinION-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples Document date: 2019_5_10
ID: hxmk6gvr_49
Snippet: To evaluate the utility of this protocol on clinical swab samples with pathogenic IBV variants, IBV-S1 gene was amplified directly from five clinical tracheal scrapings and the PCR amplicons were used to create MinION libraries. A total of 146,540 reads were successfully basecalled from the entire sequencing run (total reads = 156,000). After demultiplexing, reads per barcode ranged from 4,285 to 41,131. A total of 24,297 reads were not assigned .....
Document: To evaluate the utility of this protocol on clinical swab samples with pathogenic IBV variants, IBV-S1 gene was amplified directly from five clinical tracheal scrapings and the PCR amplicons were used to create MinION libraries. A total of 146,540 reads were successfully basecalled from the entire sequencing run (total reads = 156,000). After demultiplexing, reads per barcode ranged from 4,285 to 41,131. A total of 24,297 reads were not assigned to any of the used barcodes and 8,109 reads were discarded due to middle adapters in the basecalled reads.
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