Author: Groiss, Silvia; Pabst, Daniela; Faber, Cynthia; Meier, Andreas; Bogdoll, Annette; Unger, Conny; Nilges, Benedikt; Strauss, Sascha; Föderl-Höbenreich, Esther; Hardt, Melina; Geipel, Andreas; Reinecke, Frank; Korfhage, Christian; Zatloukal, Kurt
Title: Highly resolved spatial transcriptomics for detection of rare events in cells Cord-id: pvr5gs9p Document date: 2021_10_12
ID: pvr5gs9p
Snippet: Single-cell spatial transcriptomics technologies leveraged the potential to transcriptionally landscape sophisticated reactions in cells. Current methods to delineate such complex interplay lack the flexibility in rapid target adaptation and are particularly restricted in detecting rare transcripts. We developed a multiplex single-cell RNA In-situ hybridization technique, called ‘Molecular Cartography’ (MC) that can be easily tailored to specific applications and, by providing unprecedented
Document: Single-cell spatial transcriptomics technologies leveraged the potential to transcriptionally landscape sophisticated reactions in cells. Current methods to delineate such complex interplay lack the flexibility in rapid target adaptation and are particularly restricted in detecting rare transcripts. We developed a multiplex single-cell RNA In-situ hybridization technique, called ‘Molecular Cartography’ (MC) that can be easily tailored to specific applications and, by providing unprecedented sensitivity, specificity and resolution, is particularly suitable in tracing rare events at a subcellular level. Using a SARS-CoV-2 infection model, MC allows the discernment of single events in host-pathogen interactions, dissects primary from secondary responses, and illustrates differences in antiviral signaling pathways affected by SARS-CoV-2, simultaneously in various cell types.
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