The uteroplacental contact zone cytokine influence on NK cell cytotoxicity to trophoblasts.

Bazhenov, Dmitriy; Mikhailova, Valentina; Nikolaenkov, Igor; Markova, Kseniya; Salloum, Zeina; Kogan, Igor; Gzgzyan, Aleksandr; Selkov, Sergey; Sokolov, Dmitriy

OBJECTIVE The present study was to estimate the role of cytokines for trophoblast death in NK cells presence. METHODS This study involves assessment of NK-92 line NK cell cytotoxic activity against JEG-3 line cells, in presence of cytokines. We also assessed the effect of secretory placenta products on NK cell cytotoxic activity toward JEG-3 line cells. RESULTS Uteroplacental contact zone cytokines are able to enhance trophoblast mortality both by themselves in case of IL-1β, IL-6, IFNγ, IL-4, TGFβ, bFGF, and also through increasing the cytotoxic potential of NK cells in case of IL-1β, IFNγ, IL-8, TGFβ, and GM-CSF. PLGF decreases NK cell cytotoxicity for trophoblasts. Secretory products of first trimester placenta enhance NK cell cytotoxic potential for trophoblasts. CONCLUSIONS Cytokines of the uteroplacental contact zone can appear a mechanism ensuring trophoblast mortality dynamics throughout pregnancy.