SARS-CoV-2 is sensitive to type I interferon pretreatment

Kumari G. Lokugamage; Adam Hage; Craig Schindewolf; Ricardo Rajsbaum; Vineet D. Menachery

Tween-20) for 1 hr, and then probed with the indicated primary antibody in 3% (w/v) BSA in 304 TBST at 4°C overnight. Following overnight incubation, membranes were probed with the 305 following secondary antibodies in 5% (w/v) non-fat dry milk in TBST for 1 hr at room 306 temperature: anti-rabbit or anti-mouse IgG-HRP conjugated antibody from sheep (both 1:10,000 307 GE Healthcare). Proteins were visualized using ECL or SuperSignal West Femto 308 chemiluminescence reagents (Pierce) and detected by autoradiography. The following primary SARS-CoV WT (black) or SARS-CoV-2 (blue) at an MOI of 0.01 as described above. Each 538 point on the line graph represents the group mean, N=6 for 24 and 48HPI, N=3 for 3HPI. All 539 error bars represent SD. The two tailed students t-test was used to determine P-values: *** < 540 0.001. C) Cell protein lysates from IFN treated and untreated cells were probed 48 hours post 541 infection by using Western blotting with rabbit polyclonal anti-SARS N antibody or actin. 542 . CC-BY-NC 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.07.982264 doi: bioRxiv preprint 543 544 Figure 2 . SARS-CoV-2 infection induces STAT1 phosphorylation and ISG production. 545