Selected article for: "acceptor template and lytic spread"

Author: Hyejeong Kim; Victor D. Ellis; Andrew Woodman; Yan Zhao; Jamie J. Arnold; Craig E. Cameron
Title: RNA-dependent RNA polymerase speed and fidelity are not the only determinants of the mechanism or efficiency of recombination
  • Document date: 2019_9_14
  • ID: ljz7rfny_3
    Snippet: The one caveat of the recombination assay is that the recombinant viruses produced must be 187 able to form plaques, which may mean that the recombinant virus must spread by a lytic mechanism. It 188 was possible that PS-KH PV was defective for virus assembly and/or spread, at least lytic spread ( Fig. 189 2B). In order to score for recombinant viruses that spread by either a lytic or non-lytic mechanism, we 190 engineered the acceptor template.....
    Document: The one caveat of the recombination assay is that the recombinant viruses produced must be 187 able to form plaques, which may mean that the recombinant virus must spread by a lytic mechanism. It 188 was possible that PS-KH PV was defective for virus assembly and/or spread, at least lytic spread ( Fig. 189 2B). In order to score for recombinant viruses that spread by either a lytic or non-lytic mechanism, we 190 engineered the acceptor template to encode the UnaG (35) green fluorescent protein (Fig. 3C) yielding products that are much greater than the average length of template used in the reaction (36).

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