Selected article for: "cell toxicity and host cell"
Author: Matthew S. Faber; James T. Van Leuven; Martina M. Ederer; Yesol Sapozhnikov; Zoë L. Wilson; Holly A. Wichman; Timothy A. Whitehead; Craig R. Miller
Title: Saturation mutagenesis genome engineering of infective FX174 bacteriophage via unamplified oligo pools and golden gate assembly
  • Document date: 2019_10_9
    • ID: 02zzb7v1_22
    Snippet: Segmentation of the ΦX174 genome A phage assembly platform for ΦX174 was devised following (Donaldson et al. 2008 ). The ΦX174 chromosome was divided into 14 genomic fragments designed to avoid host cell toxicity by separating genes from their promoters and breaking large genes into multiple segments (Supplemental Note S1). Each segment is flanked by unique five nucleotide overlaps of WT ΦX174 sequence so that they can be amplified from the a.....
    Document: Segmentation of the ΦX174 genome A phage assembly platform for ΦX174 was devised following (Donaldson et al. 2008 ). The ΦX174 chromosome was divided into 14 genomic fragments designed to avoid host cell toxicity by separating genes from their promoters and breaking large genes into multiple segments (Supplemental Note S1). Each segment is flanked by unique five nucleotide overlaps of WT ΦX174 sequence so that they can be amplified from the ancestral ΦX174 using PCR primers designed to incorporate terminal BsmB1 restriction sites. Amplicons were cloned into pCR2.1 using the Invitrogen TOPO TA cloning system (Life Technologies, Grand Island, NY) and verified by Sanger sequencing.

    Search related documents:
    Co phrase search for related documents
    • assembly platform and restriction site: 1
    • clone system and host cell: 1, 2, 3
    • clone system and host cell toxicity: 1
    • clone system and restriction site: 1
    • gene separate and host cell: 1, 2, 3, 4
    • gene separate and promoter gene separate: 1, 2
    • gene separate and Sanger sequencing: 1
    • genomic fragment and host cell: 1
    • genomic fragment and PCR primer: 1, 2, 3
    • host cell and large gene: 1, 2
    • host cell and PCR primer: 1
    • host cell and promoter gene separate: 1
    • host cell and restriction site: 1
    • host cell and Sanger sequencing: 1, 2
    • host cell toxicity and restriction site: 1
    • large gene and PCR primer: 1
    • large gene and Sanger sequencing: 1
    • PCR primer and restriction site: 1, 2, 3, 4, 5
    • PCR primer and Sanger sequencing: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14