Selected article for: "generation sequencing and new virus"
Author: Hou, Tieying; Zeng, Weiqi; Yang, Minling; Chen, Wenjing; Ren, Lili; Ai, Jingwen; Wu, Ji; Liao, Yalong; Gou, Xuejing; Li, Yongjun; Wang, Xiaorui; Su, Hang; Gu, Bing; Wang, Jianwei; Xu, Teng
Title: Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19
  • Cord-id: y8c4htd4
  • Document date: 2020_8_27
    • ID: y8c4htd4
    Snippet: The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic coronavirus has raised great public health concern globally. Rapid and accurate diagnosis of this novel pathogen posts great challenges not only clinically but also technologically. Metagenomic next-generation sequencing (mNGS) and reverse-transcription PCR (RT-PCR) have been the most commonly used molecular methodologies. However, each has their own limitations. In this study, we developed an isothermal, CRISPR-bas
    Document: The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic coronavirus has raised great public health concern globally. Rapid and accurate diagnosis of this novel pathogen posts great challenges not only clinically but also technologically. Metagenomic next-generation sequencing (mNGS) and reverse-transcription PCR (RT-PCR) have been the most commonly used molecular methodologies. However, each has their own limitations. In this study, we developed an isothermal, CRISPR-based diagnostic for COVID-19 with near single-copy sensitivity. The diagnostic performances of all three technology platforms were also compared. Our study aimed to provide more insights into the molecular detection of SARS-CoV-2, and also to present a novel diagnostic option for this new emerging virus.

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