Author: Renfei Lu; Jian Wang; Min Li; Yaqi Wang; Jia Dong; Weihua Cai
Title: SARS-CoV-2 detection using digital PCR for COVID-19 diagnosis, treatment monitoring and criteria for discharge Document date: 2020_3_30
ID: mskkahwi_35
Snippet: were tested by both RT-PCR and dPCR assays. Orange color is internal reference, green is N gene, 382 blue is ORF1ab for both RT-PCR and dPCR. S1 is original specimen (left RT-PCR, right dPCR). 383 S2 is S1 specimen 5X dilution (left RT-PCR, right dPCR). S3 is S2 specimen 5X dilution (left 384 RT-PCR, right dPCR). S4 is S3 5X dilution (left RT-PCR, right dPCR). S5-S8 are 5X series 385 dilutions from S4 specimen. The dPCR assay showed at least 10 f.....
Document: were tested by both RT-PCR and dPCR assays. Orange color is internal reference, green is N gene, 382 blue is ORF1ab for both RT-PCR and dPCR. S1 is original specimen (left RT-PCR, right dPCR). 383 S2 is S1 specimen 5X dilution (left RT-PCR, right dPCR). S3 is S2 specimen 5X dilution (left 384 RT-PCR, right dPCR). S4 is S3 5X dilution (left RT-PCR, right dPCR). S5-S8 are 5X series 385 dilutions from S4 specimen. The dPCR assay showed at least 10 fold lower LLoD than RT-PCR 386 assay. RT-PCR failed to detect at S3 dilution, dPCR was able to detect S3 and S4 dilution. 387 However. LLoQ (lower limit of quantification) is above S3 concentration. 388 S1 All rights reserved. No reuse allowed without permission. author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
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