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Author: MS Zinter; CC Dvorak; MY Mayday; K Iwanaga; NP Ly; ME McGarry; GD Church; LE Faricy; CM Rowan; JR Hume; ME Steiner; ED Crawford; C Langelier; K Kalantar; ED Chow; S Miller; K Shimano; A Melton; GA Yanik; A Sapru; JL DeRisi
Title: Pulmonary Metagenomic Sequencing Suggests Missed Infections in Immunocompromised Children
  • Document date: 2018_3_29
  • ID: 28nlawnb_8
    Snippet: consisting of several open-source components (9, 10) . Briefly, reads underwent iterative removal of host (Hg38/PanTro), low quality, low complexity (LZW compression ratio >0.45), and redundant sequences using STAR, Bowtie2, PriceSeqFilter, and CD-HIT-DUP (18) (19) (20) (21) . The remaining sequences were then . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is .....
    Document: consisting of several open-source components (9, 10) . Briefly, reads underwent iterative removal of host (Hg38/PanTro), low quality, low complexity (LZW compression ratio >0.45), and redundant sequences using STAR, Bowtie2, PriceSeqFilter, and CD-HIT-DUP (18) (19) (20) (21) . The remaining sequences were then . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/291864 doi: bioRxiv preprint aligned to the NCBI non-redundant nucleotide database using GSNAPL for assignment of bacterial, fungal, viral and other taxonomic IDs (22) . Microbes were described as potentially pathogenic or typically nonpathogenic based on a priori literature review (Supplemental Text 4).

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