The Effects of 11-Ketotestosterone Implants on Transcript Levels of Gonadotropin Receptors, foxl2 and dmrt1 Genes in the Previtellogenic Ovary of Cultured Beluga (Huso huso).

Rahdari, Abdolali; Khoshkholgh, Majidreza; Yarmohammadi, Mahtab; Ortiz-Zarragoitia, Maren; Lokman, P Mark; Akhavan, Sobhan R; de Cerio, Oihane Diaz; Cancio, Ibon; Falahatkar, Bahram

The in vivo effect of 11-ketotestosterone (11KT) on transcript levels of the gonadotropin receptors (fshr and lhr) and sex differentiation-related genes (dmrt1 and foxl2) was examined in ovaries of immature female beluga. For this purpose, six fish were treated with implants containing 2.5 mg 11KT and a placebo group of six females of the same age and gametogenic stage were given a blank implant. The implants were intraperitoneally inserted into 4-year-old females at the previtellogenic stage (mean body weight 5580 ± 165 g) and maintained under culture conditions for 8 weeks. Ovary samples for gene expression analysis of lhr, fshr, dmrt1 and foxl2 were collected by biopsy at 3 and 8 weeks post-implantation. Diameters of oocytes increased in response to 11KT treatment, both at 3 and at 8 weeks post-implantation, but no obvious changes were evident in cytology. Three weeks of 11KT treatment did not affect target gene expression, but a tendency for a time-dependent decrease of lhr and dmrt1 mRNA levels was observed in both treatment and placebo groups. By 8 weeks of treatment, however, 11KT implants provoked the upregulation of fshr and foxl2 transcript levels. Furthermore, lhr and dmrt1 transcript abundances recovered by 8 weeks of exposure in both blank- and 11KT-implanted beluga. These results suggest that 11KT, either directly or indirectly, may affect gametogenesis and regulate some key components of the reproductive axis in female beluga. This article is protected by copyright. All rights reserved.