Author: Freire-Paspuel, Byron; Vega-Marino, Patricio; Velez, Alberto; Cruz, Marilyn; Bereguiain, Miguel Angel Garcia
Title: High sensitivity CDC EUA SARS-CoV-2 kit-based End Point-PCR assay Cord-id: qcirvcbf Document date: 2020_5_18
ID: qcirvcbf
Snippet: SARS-CoV-2 diagnosis is based on RT-qPCR protocols that limited testing to facilities with Real Time PCR devices and probes supply. Here we described and adapted version of the RT-qPCR CDC protocol where N1, N2 and N3 primers are used for end point PCR and amplicons are visualized on agarose gel with a limit of detection up to 20 viral RNA copies/uL. This protocol would allow to extend SARS-CoV-2 diagnosis to basic molecular biology laboratories with a great impact on surveillance programs at de
Document: SARS-CoV-2 diagnosis is based on RT-qPCR protocols that limited testing to facilities with Real Time PCR devices and probes supply. Here we described and adapted version of the RT-qPCR CDC protocol where N1, N2 and N3 primers are used for end point PCR and amplicons are visualized on agarose gel with a limit of detection up to 20 viral RNA copies/uL. This protocol would allow to extend SARS-CoV-2 diagnosis to basic molecular biology laboratories with a great impact on surveillance programs at developing countries.
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