SARS-CoV-2 susceptibility of cell lines and substrates commonly used in diagnosis and isolation of influenza and other viruses

Wang, Li; Fan, Xiaoyu; Bonenfant, Gaston; Cui, Dan; Hossain, Jaber; Jiang, Nannan; Larson, Gloria; Currier, Michael; Liddell, Jimma; Wilson, Malania; Tamin, Azaibi; Harcourt, Jennifer; Ciomperlik-Patton, Jessica; Pang, Hong; Dybdahl-Sissoko, Naomi; Campagnoli, Ray; Shi, Pei-Yong; Barnes, John; Thornburg, Natalie J.; Wentworth, David E.; Zhou, Bin

Coinfection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other viruses is inevitable as the COVID-19 pandemic continues. This study aimed to evaluate cell lines commonly used in virus diagnosis and isolation for their susceptibility to SARS-CoV-2. While multiple kidney cell lines from monkeys were susceptible and permissive to SARS-CoV-2, many cell types derived from human, dog, mink, cat, mouse, or chicken were not. Analysis of MDCK cells, which are most commonly used for surveillance and study of influenza viruses, demonstrated that they were insusceptible to SARS-CoV-2 and that the cellular barrier to productive infection was due to low expression level of the angiotensin converting enzyme 2 (ACE2) receptor and lower receptor affinity to SARS-CoV-2 spike, which could be overcome by over-expression of canine ACE2 in trans. Moreover, SARS-CoV-2 cell tropism did not appear to be affected by a D614G mutation in the spike protein.