Author: Cláudia Pereira; Rita M. Reis; José B. Gama; Dhanya K. Cheerambathur; Ana X. Carvalho; Reto Gassmann
Title: Self-assembly of the RZZ complex into filaments drives kinetochore expansion in the absence of microtubule attachment Document date: 2018_3_15
ID: ajkhpw5f_34
Snippet: cDNA coding for residues 1-591 of wild-type ZWILCH and the ZWILCH mutant E422A/D426A were cloned into a pcDNA5/FRT/TO-based vector (Invitrogen) modified to contain N-terminal Myc-GFP-TEV-S-peptide. Point mutations were subsequently introduced to make the transgene resistant to siRNA. Stable isogenic HeLa Flp-In T-REx cell lines expressing ZWILCH were generated by FRT/Flp-mediated recombination, as described previously [15] ......
Document: cDNA coding for residues 1-591 of wild-type ZWILCH and the ZWILCH mutant E422A/D426A were cloned into a pcDNA5/FRT/TO-based vector (Invitrogen) modified to contain N-terminal Myc-GFP-TEV-S-peptide. Point mutations were subsequently introduced to make the transgene resistant to siRNA. Stable isogenic HeLa Flp-In T-REx cell lines expressing ZWILCH were generated by FRT/Flp-mediated recombination, as described previously [15] .
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