Author: Cláudia Pereira; Rita M. Reis; José B. Gama; Dhanya K. Cheerambathur; Ana X. Carvalho; Reto Gassmann
Title: Self-assembly of the RZZ complex into filaments drives kinetochore expansion in the absence of microtubule attachment Document date: 2018_3_15
ID: ajkhpw5f_34
Snippet: cDNA coding for residues 1-591 of wild-type ZWILCH and the ZWILCH mutant E422A/D426A were cloned into a pcDNA5/FRT/TO-based vector (Invitrogen) modified to contain N-terminal Myc-GFP-TEV-S-peptide. Point mutations were subsequently introduced to make the transgene resistant to siRNA. Stable isogenic HeLa Flp-In T-REx cell lines expressing ZWILCH were generated by FRT/Flp-mediated recombination, as described previously [15] ......
Document: cDNA coding for residues 1-591 of wild-type ZWILCH and the ZWILCH mutant E422A/D426A were cloned into a pcDNA5/FRT/TO-based vector (Invitrogen) modified to contain N-terminal Myc-GFP-TEV-S-peptide. Point mutations were subsequently introduced to make the transgene resistant to siRNA. Stable isogenic HeLa Flp-In T-REx cell lines expressing ZWILCH were generated by FRT/Flp-mediated recombination, as described previously [15] .
Search related documents:
Co phrase search for related documents- cell line and previously describe: 1
- cell line and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- point mutation and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38
Co phrase search for related documents, hyperlinks ordered by date