Author: Michael T. Parker; Smita Gopinath; Corey E. Perez; Melissa M. Linehan; Jason M. Crawford; Akiko Iwasaki; Brett D. Lindenbach
Title: Innate Immune Priming by cGAS as a Preparatory Countermeasure Against RNA Virus Infection Document date: 2018_10_3
ID: j1gkl43g_22
Snippet: The following secondary antibodies were used for western blotting analysis: Goat To perform immunoprecipitation, lysates were pre-cleared with 2 μg/mL rabbit 472 sera and two incubations with 50 μL protein A-magnetic beads (Pierce). Samples 473 . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/434027 doi: bioRx.....
Document: The following secondary antibodies were used for western blotting analysis: Goat To perform immunoprecipitation, lysates were pre-cleared with 2 μg/mL rabbit 472 sera and two incubations with 50 μL protein A-magnetic beads (Pierce). Samples 473 . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/434027 doi: bioRxiv preprint were then rotated overnight with 2 µg HA antibody, and complexes were captured 474
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