Author: Chang Ha Woo; Sungho Jang; Giyoung Shin; Gyoo Yeol Jung; Jeong Wook Lee
Title: Sensitive one-step isothermal detection of pathogen-derived RNAs Document date: 2020_3_9
ID: 1uqjmeic_12
Snippet: We then used the ligated probe as a DNA template to test whether transcription could 122 occur. The ligation mixture was added at a 1/10 ratio to the in vitro transcription reaction 123 mixture with T7 RNA polymerase. Only when the target RNA was present in the ligation 124 reaction was the full-length transcript (92 nt) observed from transcription, thereby confirming 125 both target-dependent ligation and the subsequent transcription (Fig. 2b) ......
Document: We then used the ligated probe as a DNA template to test whether transcription could 122 occur. The ligation mixture was added at a 1/10 ratio to the in vitro transcription reaction 123 mixture with T7 RNA polymerase. Only when the target RNA was present in the ligation 124 reaction was the full-length transcript (92 nt) observed from transcription, thereby confirming 125 both target-dependent ligation and the subsequent transcription (Fig. 2b) . 126
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