Selected article for: "pot step and RNA detection"

Author: Chang Ha Woo; Sungho Jang; Giyoung Shin; Gyoo Yeol Jung; Jeong Wook Lee
Title: Sensitive one-step isothermal detection of pathogen-derived RNAs
  • Document date: 2020_3_9
  • ID: 1uqjmeic_36
    Snippet: The simple enzyme composition is another reason to enable one-step and one-pot 311 detection. The fewer the enzymes, the easier it is to optimize in terms of temperature and 312 buffer composition. In designing the detection scheme, we deliberately tried to reduce the 313 number of enzymes, thus creating one of the simplest isothermal detection schemes based on 314 two enzymes: SplintR ligase for target detection and T7 RNA polymerase for amplifi.....
    Document: The simple enzyme composition is another reason to enable one-step and one-pot 311 detection. The fewer the enzymes, the easier it is to optimize in terms of temperature and 312 buffer composition. In designing the detection scheme, we deliberately tried to reduce the 313 number of enzymes, thus creating one of the simplest isothermal detection schemes based on 314 two enzymes: SplintR ligase for target detection and T7 RNA polymerase for amplification. 315

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