Author: Giadone, Richard M.; Mithal, Aditya; Miller, Gregory J.; Matte, Taylor M.; Yeung, Anthony K.; Dowrey, Todd W.; Werder, Rhiannon B.; Miller, Nancy S.; Andry, Christopher D.; Vanuytsel, Kim; Murphy, George J.
Title: qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples Cord-id: qlflyq5s Document date: 2020_9_15
ID: qlflyq5s
Snippet: The protocols herein outline the use of qRT-PCR to detect the presence of SARS-CoV-2 genomic RNA in patient samples. In order to cope with potential fluctuations in supply chain and testing demands and to enable expedient adaptation of reagents and assays on hand, we include details for three parallel methodologies (one- and two-step singleplex and one-step multiplex assays). The diagnostic platforms described can be easily adapted by basic science research laboratories for SARS-CoV-2 diagnostic
Document: The protocols herein outline the use of qRT-PCR to detect the presence of SARS-CoV-2 genomic RNA in patient samples. In order to cope with potential fluctuations in supply chain and testing demands and to enable expedient adaptation of reagents and assays on hand, we include details for three parallel methodologies (one- and two-step singleplex and one-step multiplex assays). The diagnostic platforms described can be easily adapted by basic science research laboratories for SARS-CoV-2 diagnostic testing with relatively short turnaround time. For complete details on the use and execution of this protocol, please refer to Vanuytsel et al. (2020).
Search related documents:
Co phrase search for related documents- accurate reporting and administration drug food: 1
- accurate reporting and local hospital: 1
- accurate reporting and lod detection limit: 1
- accurate reporting and lod determine: 1
- administration drug and local hospital: 1, 2
- administration drug and lod detection limit: 1, 2, 3, 4, 5, 6, 7, 8, 9
- administration drug and lod determine: 1
- administration drug and long term storage: 1
- administration drug food and local hospital: 1, 2
- administration drug food and lod detection limit: 1, 2, 3, 4, 5, 6, 7, 8, 9
- administration drug food and lod determine: 1
- administration drug food and long term storage: 1
Co phrase search for related documents, hyperlinks ordered by date