Selected article for: "cdna library and library construction"
Author: Chin-Yi Chu; Xing Qiu; Matthew N. McCall; Lu Wang; Anthony Corbett; Jeanne Holden-Wiltse; Christopher Slaunwhite; Qian Wang; Christopher Anderson; Alex Grier; Steven R. Gill; Gloria S. Pryhuber; Ann R. Falsey; David J. Topham; Mary T. Caserta; Edward E. Walsh; Thomas J Mariani
Title: Insufficiency in airway interferon activation defines clinical severity to infant RSV infection
  • Document date: 2019_5_20
    • ID: bx49tbui_22
    Snippet: The RNA was isolated and assessed for quality and quantity (average yield 275 ng per sample). 1 ng of total RNA was amplified using the SMARter Ultra Low amplification kit (Clontech, Mountain, CA) with PCR amplification to generate sufficient cDNA for library construction. Amplified cDNA quantification was determined with the Qubit Flourometer (Life Technologies, Grand Island, NY) and quality was assessed using the Agilent Bioanalyzer 2100 (Santa.....
    Document: The RNA was isolated and assessed for quality and quantity (average yield 275 ng per sample). 1 ng of total RNA was amplified using the SMARter Ultra Low amplification kit (Clontech, Mountain, CA) with PCR amplification to generate sufficient cDNA for library construction. Amplified cDNA quantification was determined with the Qubit Flourometer (Life Technologies, Grand Island, NY) and quality was assessed using the Agilent Bioanalyzer 2100 (Santa Clara, CA). Library construction was performed using NexteraXT library kit (Illumina, San Diego, CA) with 1 ng of input amplified cDNA per manufacturer's recommendations. Nextera libraries were quantified with the Qubit Flourometer (Life Technologies, Grand Island, NY) and quality was assessed using the Agilent Tape Station (Santa Clara, CA). Libraries were sequenced on the Illumina HiSeq2500 (Illumina, San Diego, CA) to generate 20 million 1X100-bp single end reads per sample 35 .

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