Selected article for: "HRP conjugation and IgG detection"
Author: Xiaotian Tan; Cory Lin; Jie Zhang; Maung Kyaw Khaing Oo; Xudong Fan
Title: Rapid and quantitative detection of COVID-19 markers in micro-liter sized samples
  • Document date: 2020_4_22
    • ID: 0tdfvlqd_10
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.20.052233 doi: bioRxiv preprint Biological (Catalog number: 40150-D001, 40150-D003, and 40150-D006). The anti-polyhistidine antibody that was used in polyhistidine-mediated S1 protein immobilization (see Figure S1 ) was purchased from Thermo Fisher (MA1-21315). The horseradish peroxide (HRP) conjugated secondary antibody used in the IgG d.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.20.052233 doi: bioRxiv preprint Biological (Catalog number: 40150-D001, 40150-D003, and 40150-D006). The anti-polyhistidine antibody that was used in polyhistidine-mediated S1 protein immobilization (see Figure S1 ) was purchased from Thermo Fisher (MA1-21315). The horseradish peroxide (HRP) conjugated secondary antibody used in the IgG detection experiment was from the detection antibody in Thermo Fisher's human total IgG ELISA kit (88-50550-22). The HRP conjugation of CR3022, D001, D003, and D006 antibodies were carried out with Abcam's HRP conjugation kit (ab102890) with a molar ratio of antibody : HRP = 1 : 4.

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