Author: Sun, Fu; Ganguli, Anurup; Nguyen, Judy; Brisbin, Ryan; Shanmugam, Krithika; Hirschberg, David L; Wheeler, Matthew B; Bashir, Rashid; Nash, David M; Cunningham, Brian T
Title: Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract. Cord-id: ytr7fi0g Document date: 2020_4_25
ID: ytr7fi0g
Snippet: Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microf
Document: Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes.
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