Rapid and quantitative detection of COVID-19 markers in micro-liter sized samples

Xiaotian Tan; Cory Lin; Jie Zhang; Maung Kyaw Khaing Oo; Xudong Fan

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.20.052233 doi: bioRxiv preprint second and the third antibodies, D001 and D006, are humanized chimeric IgGs (the precursors of D001 and D006 were originally raised in mouse and rabbit, respectively) that are specific to the S1 protein of SARS-CoV. Based on the preliminary results conducted at Sino Biological internally, they were also believed to have high binding affinities towards the S1 protein of SARS-CoV-2. In order to mimic the actual clinical situations, we decided to use 50 times diluted human serum as the solvent of the IgG antibodies (50-1000 are the typical dilution factors of serum in actual serological analyses). To evaluate the differences in antibody's affinity towards SARS-CoV-2 S1