Selected article for: "crystal structure and macro domain"
Author: Cho, Chao-Cheng; Lin, Meng-Hsuan; Chuang, Chien-Ying; Hsu, Chun-Hua
Title: Macro Domain from Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Is an Efficient ADP-ribose Binding Module: CRYSTAL STRUCTURE AND BIOCHEMICAL STUDIES
  • Cord-id: uq2idlca
  • Document date: 2016_1_5
    • ID: uq2idlca
    Snippet: The newly emerging Middle East respiratory syndrome coronavirus (MERS-CoV) encodes the conserved macro domain within non-structural protein 3. However, the precise biochemical function and structure of the macro domain is unclear. Using differential scanning fluorimetry and isothermal titration calorimetry, we characterized the MERS-CoV macro domain as a more efficient adenosine diphosphate (ADP)-ribose binding module than macro domains from other CoVs. Furthermore, the crystal structure of the
    Document: The newly emerging Middle East respiratory syndrome coronavirus (MERS-CoV) encodes the conserved macro domain within non-structural protein 3. However, the precise biochemical function and structure of the macro domain is unclear. Using differential scanning fluorimetry and isothermal titration calorimetry, we characterized the MERS-CoV macro domain as a more efficient adenosine diphosphate (ADP)-ribose binding module than macro domains from other CoVs. Furthermore, the crystal structure of the MERS-CoV macro domain was determined at 1.43-Å resolution in complex with ADP-ribose. Comparison of macro domains from MERS-CoV and other human CoVs revealed structural differences in the α1 helix alters how the conserved Asp-20 interacts with ADP-ribose and may explain the efficient binding of the MERS-CoV macro domain to ADP-ribose. This study provides structural and biophysical bases to further evaluate the role of the MERS-CoV macro domain in the host response via ADP-ribose binding but also as a potential target for drug design.

    Search related documents:
    Co phrase search for related documents
    • adp ribose and liver disease: 1
    • adp ribose and liver pathology: 1
    • adp ribose and macro domain: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31
    • adp ribose and macro domain adp ribose binding: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • adp ribose and macro domain role: 1, 2, 3
    • adp ribose bind ability and macro domain: 1
    • adp ribose bind ability and macro domain adp ribose binding: 1
    • adp ribose bind affinity and macro domain: 1
    • adp ribose bind and adrp activity: 1, 2, 3, 4, 5
    • adp ribose bind and macro domain: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • adp ribose bind and macro domain adp ribose binding: 1, 2
    • adp ribose binding and adrp activity: 1, 2, 3, 4
    • adp ribose binding and liver disease: 1
    • adp ribose binding and liver pathology: 1
    • liver disease and loop region: 1
    • liver disease and macro domain: 1
    • liver disease and macro domain adp ribose binding: 1