Author: Li, Yang; Lai, Dan-yun; Tao, Sheng-ce
Title: SARS-CoV-2 Spike linear epitope scanning via a peptide microarray through sera profiling Cord-id: yyie09ib Document date: 2021_7_10
ID: yyie09ib
Snippet: Host humoral immunological response plays an essential role in protection against pathogens. Identification- of B cell epitopes on antigens is required for accurate diagnosis and vaccine development. To map SARS-CoV-2 Spike linear epitopes, we developed a protocol of profiling sera from COVID-19 patients via a peptide microarray designed according to Spike protein. The protocol is also applicable for other antigens or sample types. This protocol is rapid, high-throughput and the cost is acceptab
Document: Host humoral immunological response plays an essential role in protection against pathogens. Identification- of B cell epitopes on antigens is required for accurate diagnosis and vaccine development. To map SARS-CoV-2 Spike linear epitopes, we developed a protocol of profiling sera from COVID-19 patients via a peptide microarray designed according to Spike protein. The protocol is also applicable for other antigens or sample types. This protocol is rapid, high-throughput and the cost is acceptable while it needs specialized microarray facilities.
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