Selected article for: "dPCR assay and negative result"
Author: Renfei Lu; Jian Wang; Min Li; Yaqi Wang; Jia Dong; Weihua Cai
Title: SARS-CoV-2 detection using digital PCR for COVID-19 diagnosis, treatment monitoring and criteria for discharge
  • Document date: 2020_3_30
    • ID: mskkahwi_22
    Snippet: Stool specimen from patient 4 on Feb. 24 th was tested as negative by both However, the internal reference gene result by dPCR is 0 suggesting no RNA was loaded in either tests 227 or the PCR reactions were inhibited. The samples need to be re-collected to re-run the tests. The 228 internal reference gene of dPCR assay serves as a quality control to ensures no PCR inhibition 229 happened and RNA extraction was successful. Without such a control i.....
    Document: Stool specimen from patient 4 on Feb. 24 th was tested as negative by both However, the internal reference gene result by dPCR is 0 suggesting no RNA was loaded in either tests 227 or the PCR reactions were inhibited. The samples need to be re-collected to re-run the tests. The 228 internal reference gene of dPCR assay serves as a quality control to ensures no PCR inhibition 229 happened and RNA extraction was successful. Without such a control in RT-PCR assay, the patient 230 could have been discharged prematurely, putting those in close contact at risk of being infected. 231 232

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