Selected article for: "dPCR assay and serial dilution"
Author: Renfei Lu; Jian Wang; Min Li; Yaqi Wang; Jia Dong; Weihua Cai
Title: SARS-CoV-2 detection using digital PCR for COVID-19 diagnosis, treatment monitoring and criteria for discharge
  • Document date: 2020_3_30
    • ID: mskkahwi_24
    Snippet: We first demonstrated that the LOD of dPCR assay is at least 10 times better than that of RT-PCR 234 assay using serial dilution of the same clinical sample. Higher sensitivity, along with more reliable 235 quantification of viral load, provides valuable information to help clinicians choose the appropriate 236 treatment plan 16 . To demonstrate its application in clinical settings, we performed head-to-head 237 comparison of RT-PCR and dPCR assa.....
    Document: We first demonstrated that the LOD of dPCR assay is at least 10 times better than that of RT-PCR 234 assay using serial dilution of the same clinical sample. Higher sensitivity, along with more reliable 235 quantification of viral load, provides valuable information to help clinicians choose the appropriate 236 treatment plan 16 . To demonstrate its application in clinical settings, we performed head-to-head 237 comparison of RT-PCR and dPCR assays using a cohort of 39 patients totaling 109 samples obtained 238 at different stage of the treatment and from different locations.. 239

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