Author: Neha Jain; Uma Shankar; Prativa Majee; Amit Kumar
Title: Scrutinizing the SARS-CoV-2 protein information for the designing an effective vaccine encompassing both the T-cell and B-cell epitopes Document date: 2020_4_1
ID: lmstdmyb_40
Snippet: Escherichia coli K12 satrain, the construct was first converted to cDNA using Reverse translate tool available at Expasy server. The resultant DNA was further optimized for enhanced protein expression by using JCAT server [55] . Finally, the cDNA construct was inserted into the pET28a (+) vector using HindIII and BamHI restriction sites......
Document: Escherichia coli K12 satrain, the construct was first converted to cDNA using Reverse translate tool available at Expasy server. The resultant DNA was further optimized for enhanced protein expression by using JCAT server [55] . Finally, the cDNA construct was inserted into the pET28a (+) vector using HindIII and BamHI restriction sites.
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